4V4I

Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements.

This is a non-PDB format compatible entry.

Replaces:  1VS9Replaces:  2I1C

Summary for 4V4I

• Entry DOI: 10.2210/pdb4v4i/pdb
• Related: 1GIX 1GIY 2OW8
• Descriptor: 23S LARGE SUBUNIT RIBOSOMAL RNA, 50S ribosomal protein L13, 50S ribosomal protein L14, ... (54 entities in total)
• Functional Keywords: ribosome, functional complex, trna, e site, p site, peptidyl transferase center
• Biological source: Thermus thermophilus; More
• Total number of polymer chains: 54
• Total formula weight: 2224699.39

Authors

Korostelev, A.,Trakhanov, S.,Laurberg, M.,Noller, H.F. (deposition date: 2007-02-15, release date: 2014-07-09, Last modification date: 2024-10-30)

Primary citation

Korostelev, A.,Trakhanov, S.,Laurberg, M.,Noller, H.F.
Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements
Cell(Cambridge,Mass.), 126:1065-1077, 2006

PubMed Abstract: Our understanding of the mechanism of protein synthesis has undergone rapid progress in recent years as a result of low-resolution X-ray and cryo-EM structures of ribosome functional complexes and high-resolution structures of ribosomal subunits and vacant ribosomes. Here, we present the crystal structure of the Thermus thermophilus 70S ribosome containing a model mRNA and two tRNAs at 3.7 A resolution. Many structural details of the interactions between the ribosome, tRNA, and mRNA in the P and E sites and the ways in which tRNA structure is distorted by its interactions with the ribosome are seen. Differences between the conformations of vacant and tRNA-bound 70S ribosomes suggest an induced fit of the ribosome structure in response to tRNA binding, including significant changes in the peptidyl-transferase catalytic site.

PubMed: 16962654
DOI: 10.1016/j.cell.2006.08.032

Experimental method

X-RAY DIFFRACTION (3.71 Å)