4V4I
Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements.
This is a non-PDB format compatible entry.
Replaces: 1VS9Replaces: 2I1CExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 507.810, 507.810, 689.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.710 |
| R-factor | 0.349 |
| Rwork | 0.348 |
| R-free | 0.35300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1TWT AND 1TWV |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.393 |
| Data reduction software | d*TREK |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.149 | 0.467 |
| Number of reflections | 465894 | |
| Completeness [%] | 99.3 | 96.6 |
| Redundancy | 5.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | MPD | ||
| 4 | 1 | 2 | H2O |
