4UER

40S-eIF1-eIF1A-eIF3-eIF3j translation initiation complex from Lachancea kluyveri

Summary for 4UER

Entry DOI	10.2210/pdb4uer/pdb
EMDB information	2845
Descriptor	EIF1A, ES31, 18S RRNA, ... (40 entities in total)
Functional Keywords	eif3, translation initiation, translation
Biological source	LACHANCEA KLUYVERI More
Total number of polymer chains	39
Total formula weight	1428954.99
Authors	Aylett, C.H.S.,Boehringer, D.,Erzberger, J.P.,Schaefer, T.,Ban, N. (deposition date: 2014-12-18, release date: 2015-02-11, Last modification date: 2024-05-08)
Primary citation	Aylett, C.H.S.,Boehringer, D.,Erzberger, J.P.,Schaefer, T.,Ban, N. Structure of a Yeast 40S-Eif1-Eif1A-Eif3-Eif3J Initiation Complex Nat.Struct.Mol.Biol., 22:269-, 2015 Cited by PubMed Abstract: Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits. PubMed: 25664723 DOI: 10.1038/NSMB.2963 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (6.47 Å)

Structure validation