4UER
40S-eIF1-eIF1A-eIF3-eIF3j translation initiation complex from Lachancea kluyveri
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.47 |
| Software | CTFFIND,UCSF Chimera,RELION |
| Symmetry | C1 |
