4U2F

Crystal structure of dienelactone hydrolase B-1 variant (Q35H, F38L, Y64H, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.80 A resolution

Summary for 4U2F

Related4U2B 4U2C 4U2D 4U2E 4U2G
DescriptorCarboxymethylenebutenolidase, SULFATE ION (3 entities in total)
Functional Keywordshydrolase, a/b hydrolase fold
Biological sourcePseudomonas sp.
Total number of polymer chains1
Total molecular weight25278.57
Authors
Porter, J.L.,Collyer, C.A.,Ollis, D.L. (deposition date: 2014-07-16, release date: 2014-12-10, Last modification date: 2020-01-01)
Primary citation
Porter, J.L.,Boon, P.L.,Murray, T.P.,Huber, T.,Collyer, C.A.,Ollis, D.L.
Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search.
Acs Chem.Biol., 10:611-621, 2015
PubMed: 25419863 (PDB entries with the same primary citation)
DOI: 10.1021/cb500809f
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.8 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.223401.1%4.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
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PDB entries from 2020-07-29