4U2F
Crystal structure of dienelactone hydrolase B-1 variant (Q35H, F38L, Y64H, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.80 A resolution
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Carboxymethylenebutenolidase | polymer | 236 | 25182.5 | 1 | UniProt (P0A115) Pfam (PF01738) In PDB | Pseudomonas sp. | Dienelactone hydrolase,DLH |
2 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
3 | water | water | 18.0 | 173 | Chemie (HOH) |
Sequence modifications
A: 1 - 236 (UniProt: P0A115)
PDB | External Database | Details |
---|---|---|
His 35 | Gln 35 | engineered mutation |
Leu 38 | Phe 38 | engineered mutation |
His 64 | Tyr 64 | engineered mutation |
Ala 79 | Arg 79 | conflict |
Leu 110 | Gln 110 | engineered mutation |
Ser 123 | Cys 123 | engineered mutation |
Cys 137 | Tyr 137 | engineered mutation |
Cys 145 | Tyr 145 | engineered mutation |
Asp 154 | Lys 154 | engineered mutation |
Gly 199 | Glu 199 | engineered mutation |
Gly 208 | Ser 208 | engineered mutation |
Asp 211 | Gly 211 | engineered mutation |
Thr 224 | Arg 224 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 25182.5 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 96.1 | |
All* | Total formula weight | 25278.6 |