Lac repressor engineered to bind sucralose, unliganded tetramer

Summary for 4RZS

DescriptorLac repressor, GLYCEROL (3 entities in total)
Functional Keywordssucralose, laci, lac repressor, lactose operon repressor, protein design, allostery, escherichia coli, transcription
Biological sourceEscherichia coli
Total number of polymer chains4
Total molecular weight163902.33
Arbing, M.A.,Cascio, D.,Kosuri, S.,Church, G.M. (deposition date: 2014-12-24, release date: 2015-12-23, Last modification date: 2017-11-22)
Primary citation
Taylor, N.D.,Garruss, A.S.,Moretti, R.,Chan, S.,Arbing, M.A.,Cascio, D.,Rogers, J.K.,Isaacs, F.J.,Kosuri, S.,Baker, D.,Fields, S.,Church, G.M.,Raman, S.
Engineering an allosteric transcription factor to respond to new ligands.
Nat.Methods, 13:177-183, 2016
PubMed: 26689263 (PDB entries with the same primary citation)
DOI: 10.1038/nmeth.3696
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.23751.6%6.9%4.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload