4RZS

Lac repressor engineered to bind sucralose, unliganded tetramer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Lac repressor	polymer	381	40883.5	4	UniProt (C9QQT3) Pfam (PF00356) Pfam (PF13377) UniProt (by SIFTS) (P03023)	Escherichia coli	Transcriptional regulator, LacI family
2	E, F, G, H (A, B, C, D)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
3	I, J, K, L (A, B, C, D)	water	water		18.0	23	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 360 (UniProt: C9QQT3)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Thr 149	Asp 152	engineered mutation
Ala 150	Val 153	engineered mutation
Leu 156	Ile 159	engineered mutation
Asp 193	Ser 196	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	163534.0
Non-Polymers*	Number of molecules	4
	Total formula weight	368.4
All*	Total formula weight	163902.3

*Water molecules are not included.