4RZS

Lac repressor engineered to bind sucralose, unliganded tetramer

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000976	molecular_function	transcription cis-regulatory region binding
A	0000987	molecular_function	cis-regulatory region sequence-specific DNA binding
A	0001217	molecular_function	DNA-binding transcription repressor activity
A	0003677	molecular_function	DNA binding
A	0003700	molecular_function	DNA-binding transcription factor activity
A	0005829	cellular_component	cytosol
A	0006355	biological_process	regulation of DNA-templated transcription
A	0042802	molecular_function	identical protein binding
A	0045892	biological_process	negative regulation of DNA-templated transcription
B	0000976	molecular_function	transcription cis-regulatory region binding
B	0000987	molecular_function	cis-regulatory region sequence-specific DNA binding
B	0001217	molecular_function	DNA-binding transcription repressor activity
B	0003677	molecular_function	DNA binding
B	0003700	molecular_function	DNA-binding transcription factor activity
B	0005829	cellular_component	cytosol
B	0006355	biological_process	regulation of DNA-templated transcription
B	0042802	molecular_function	identical protein binding
B	0045892	biological_process	negative regulation of DNA-templated transcription
C	0000976	molecular_function	transcription cis-regulatory region binding
C	0000987	molecular_function	cis-regulatory region sequence-specific DNA binding
C	0001217	molecular_function	DNA-binding transcription repressor activity
C	0003677	molecular_function	DNA binding
C	0003700	molecular_function	DNA-binding transcription factor activity
C	0005829	cellular_component	cytosol
C	0006355	biological_process	regulation of DNA-templated transcription
C	0042802	molecular_function	identical protein binding
C	0045892	biological_process	negative regulation of DNA-templated transcription
D	0000976	molecular_function	transcription cis-regulatory region binding
D	0000987	molecular_function	cis-regulatory region sequence-specific DNA binding
D	0001217	molecular_function	DNA-binding transcription repressor activity
D	0003677	molecular_function	DNA binding
D	0003700	molecular_function	DNA-binding transcription factor activity
D	0005829	cellular_component	cytosol
D	0006355	biological_process	regulation of DNA-templated transcription
D	0042802	molecular_function	identical protein binding
D	0045892	biological_process	negative regulation of DNA-templated transcription

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 501

Chain	Residue
A	ARG197
A	ASN246
A	ASP274
A	GLN291

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL B 501

Chain	Residue
B	ARG197
B	ASN246
B	ASP274
B	GLN291

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL C 501

Chain	Residue
C	ARG197
C	ASN246
C	ASP274
C	ASP193

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL D 501

Chain	Residue
D	ASP193
D	ARG197
D	ASP274
D	GLN291

---

Functional Information from PROSITE/UniProt

site_id	PS00356
Number of Residues	19
Details	HTH_LACI_1 LacI-type HTH domain signature. LyDVAeyAgVSyqTVsrVV

Chain	Residue	Details
A	LEU6-VAL24

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	38
Details	DNA binding: {"description":"H-T-H motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU00111","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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