Crystal structure of the Middle-East respiratory syndrome coronavirus papain-like protease in complex with ubiquitin (space group P6522)

Summary for 4RF0

Related4REZ 4RF1
DescriptorORF1ab protein, Ubiquitin-60S ribosomal protein L40, ZINC ION, ... (6 entities in total)
Functional Keywordszinc ribbon, deubiquitinase, papain-like protease, protein binding
Biological sourceHuman betacoronavirus 2c Jordan-N3/2012
Cellular locationHost cytoplasm, host perinuclear region . Host membrane ; Multi-pass membrane protein  M4STU1
Ubiquitin: Cytoplasm . 60S ribosomal protein L40: Cytoplasm  P62987
Total number of polymer chains2
Total molecular weight45555.33
Bailey-Elkin, B.A.,Johnson, G.G.,Mark, B.L. (deposition date: 2014-09-24, release date: 2014-10-22, Last modification date: 2015-01-14)
Primary citation
Bailey-Elkin, B.A.,Knaap, R.C.,Johnson, G.G.,Dalebout, T.J.,Ninaber, D.K.,van Kasteren, P.B.,Bredenbeek, P.J.,Snijder, E.J.,Kikkert, M.,Mark, B.L.
Crystal Structure of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Papain-like Protease Bound to Ubiquitin Facilitates Targeted Disruption of Deubiquitinating Activity to Demonstrate Its Role in Innate Immune Suppression.
J.Biol.Chem., 289:34667-34682, 2014
PubMed: 25320088 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M114.609644
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.282404.0%4.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report