4RF0

Crystal structure of the Middle-East respiratory syndrome coronavirus papain-like protease in complex with ubiquitin (space group P6522)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Wavelength(s)	1.28219
Spacegroup name	P 65 2 2
Unit cell lengths	176.972, 176.972, 84.549
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.240 - 2.800
R-factor	0.2431
Rwork	0.240
R-free	0.28100
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.539
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.240
High resolution limit [Å]	2.600
Number of reflections	24473
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1.80 M ammonium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K