4QQZ

Crystal structure of T. fusca Cas3-AMPPNP

Summary for 4QQZ

Related4QQX 4QQY 4QQZ
DescriptorCRISPR-associated helicase, Cas3 family, DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3'), FE (III) ION, ... (4 entities in total)
Functional Keywordshydrolase, helicase, hydrolase-dna complex, hydrolase/dna
Biological sourceThermobifida fusca
Total number of polymer chains8
Total molecular weight442168.08
Authors
Ke, A.,Huo, Y.,Nam, K.H. (deposition date: 2014-06-30, release date: 2014-08-27, Last modification date: 2014-09-24)
Primary citation
Huo, Y.,Nam, K.H.,Ding, F.,Lee, H.,Wu, L.,Xiao, Y.,Farchione, M.D.,Zhou, S.,Rajashankar, K.,Kurinov, I.,Zhang, R.,Ke, A.
Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation.
Nat.Struct.Mol.Biol., 21:771-777, 2014
PubMed: 25132177 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.2875
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.93 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.232110.1%10.6%0.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4qqz
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Molmil generated image of 4qqz
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Molmil generated image of 4qqz
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 4qqz
no rotation
Molmil generated image of 4qqz
rotated about x axis by 90°
Molmil generated image of 4qqz
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (4qqz.pdb3.gz [152.77 KB])
Coordinate files for Biological unit (4qqz.pdb4.gz [151.25 KB])
Coordinate files for Biological unit (4qqz.pdb6.gz [294.64 KB])
Coordinate files for Biological unit (4qqz.pdb2.gz [151.93 KB])
Coordinate files for Biological unit (4qqz.pdb5.gz [295.53 KB])
Coordinate files for Biological unit (4qqz.pdb1.gz [153.01 KB])