4QQZ

Crystal structure of T. fusca Cas3-AMPPNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	CRISPR-associated helicase, Cas3 family	polymer	964	106210.6	4	UniProt (Q47PJ0) Pfam (PF18019) Pfam (PF22590) In PDB	Thermobifida fusca
2	B, D, F, H	DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3')	polymer	12	3713.5	4
3	A, C, E, G	FE (III) ION	non-polymer		55.8	8	Chemie (FE)
4	A, C, E, G	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	4	Chemie (ANP)

Sequence modifications

A, C, E, G: 1 - 944 (UniProt: Q47PJ0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	439696.5
Non-Polymers*	Number of molecules	12
	Total formula weight	2471.5
All*	Total formula weight	442168.1

*Water molecules are not included.