4QIL

Crystal structure of the ROQ domain of human Roquin in complex with the Hmg19 stem-loop RNA

4QIL の概要

• エントリーDOI: 10.2210/pdb4qil/pdb
• 関連するPDBエントリー: 4QIK
• 分子名称: Roquin-1, 5'-R(*CP*UP*CP*CP*CP*UP*UP*CP*UP*GP*UP*GP*AP*AP*GP*GP*GP*GP*A)-3', CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: winged-helix motif, mrna stem-loop, mrna decay, immune responses, autoimmunity, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, P-body (By similarity): Q5TC82
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 86650.29

構造登録者

Tan, D.,Tong, L. (登録日: 2014-05-31, 公開日: 2014-07-16, 最終更新日: 2023-09-20)

主引用文献

Tan, D.,Zhou, M.,Kiledjian, M.,Tong, L.
The ROQ domain of Roquin recognizes mRNA constitutive-decay element and double-stranded RNA.
Nat.Struct.Mol.Biol., 21:679-685, 2014

PubMed Abstract: A conserved stem-loop motif of the constitutive decay element (CDE) in the 3' UTR of mRNAs is recognized by the ROQ domain of Roquin, which mediates mRNA degradation. Here we report two crystal structures of the Homo sapiens ROQ domain in complex with CDE RNA. The ROQ domain has an elongated shape with three subdomains. The 19-nt Hmgxb3 CDE is bound as a stem-loop to domain III. The 23-nt TNF RNA is bound as a duplex to a separate site at the interface between domains I and II. Mutagenesis studies confirm that the ROQ domain has two separate RNA-binding sites, one for stem-loop RNA (A site) and the other for double-stranded RNA (B site). Mutation in either site perturbs the Roquin-mediated degradation of HMGXB3 and IL6 mRNAs in human cells, demonstrating the importance of both sites for mRNA decay.

PubMed: 25026078
DOI: 10.1038/nsmb.2857

実験手法

X-RAY DIFFRACTION (2.9 Å)