4PWK

Crystal structure of V30M mutant human transthyretin complexed with dihydroguaiaretic acid

Summary for 4PWK

• Entry DOI: 10.2210/pdb4pwk/pdb
• Related: 4PWE 4PWF 4PWG 4PWH 4PWI 4PWJ
• Descriptor: Transthyretin, 4,4'-[(2R,3R)-2,3-dimethylbutane-1,4-diyl]bis(2-methoxyphenol) (3 entities in total)
• Functional Keywords: transporter, thyroxine binding, transport protein
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P02766
• Total number of polymer chains: 2
• Total formula weight: 35346.00

Authors

Yokoyama, T.,Kosaka, Y.,Mizuguchi, M. (deposition date: 2014-03-20, release date: 2014-11-26, Last modification date: 2023-11-08)

Primary citation

Yokoyama, T.,Kosaka, Y.,Mizuguchi, M.
Inhibitory Activities of Propolis and Its Promising Component, Caffeic Acid Phenethyl Ester, against Amyloidogenesis of Human Transthyretin
J.Med.Chem., 57:8928-8935, 2014

PubMed Abstract: Transthyretin (TTR) is a homotetrameric serum protein associated with amyloidoses such as familial amyloid polyneuropathy and senile systemic amyloidosis. The amyloid fibril formation of TTR can be inhibited through stabilization of the TTR tetramer by the binding of small molecules. In this study, we examined the inhibitory potency of caffeic acid phenethyl ester (CAPE) and its derivatives. Thioflavin T assay showed that CAPE suppressed the amyloid fibril formation of TTR. Comparative analysis of the inhibitory potencies revealed that phenethyl ferulate was the most potent among the CAPE derivatives. The binding of phenethyl ferulate and the selected compounds to TTR were confirmed by the 8-anilino-1-naphthalenesulfonic acid displacement and X-ray crystallography. It was also demonstrated that Bio 30, which is a CAPE-rich commercially available New Zealand propolis, inhibited TTR amyloidogenesis and stabilized the TTR tetramer. These results suggested that a propolis may be efficient for preventing TTR amyloidosis.

PubMed: 25314129
DOI: 10.1021/jm500997m

Experimental method

X-RAY DIFFRACTION (1.59 Å)