4OL8

Ty3 reverse transcriptase bound to DNA/RNA

Summary for 4OL8

DescriptorReverse transcriptase/ribonuclease H, 5'-R(*CP*UP*GP*AP*GP*AP*GP*AP*GP*AP*GP*GP*AP*AP*GP*AP*UP*G)-3', 5'-D(*CP*AP*TP*CP*TP*TP*CP*CP*TP*CP*TP*CP*TP*CP*TP*C)-3', ... (5 entities in total)
Functional Keywordsprotein-dna/rna, reverse transcription, dna/rna binding, transferase, hydrolase-rna-dna complex, hydrolase/rna/dna
Biological sourceSaccharomyces cerevisiae (Baker's yeast)
Cellular locationCytoplasm Q99315
Total number of polymer chains8
Total molecular weight241729.32
Authors
Nowak, E.,Miller, J.T.,Bona, M.K.,Studnicka, J.,Szczepanowski, R.H.,Jurkowski, J.,Le Grice, S.F.J.,Nowotny, M. (deposition date: 2014-01-23, release date: 2014-03-05, Last modification date: 2014-04-16)
Primary citation
Nowak, E.,Miller, J.T.,Bona, M.K.,Studnicka, J.,Szczepanowski, R.H.,Jurkowski, J.,Le Grice, S.F.,Nowotny, M.
Ty3 reverse transcriptase complexed with an RNA-DNA hybrid shows structural and functional asymmetry.
Nat.Struct.Mol.Biol., 21:389-396, 2014
PubMed: 24608367 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.2785
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.1 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.296110.9%11.8%4.4%0.61MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution