4OL8
Ty3 reverse transcriptase bound to DNA/RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 320.700, 75.062, 108.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.898 - 3.100 |
R-factor | 0.2305 |
Rwork | 0.227 |
R-free | 0.29560 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.069 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.898 | 3.290 |
High resolution limit [Å] | 2.999 | 2.999 |
Rmerge | 0.102 | 0.847 |
Number of reflections | 53285 | |
<I/σ(I)> | 11.9 | 2.1 |
Completeness [%] | 99.6 | 98.4 |
Redundancy | 5.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M Tris, 0.2 M ammonium sulfate, 17% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |