4NIY

Crystal structure of trypsiligase (K60E/N143H/Y151H/D189K trypsin) complexed to YRH-ecotin (M84Y/M85R/A86H ecotin)

Summary for 4NIY

Entry DOI	10.2210/pdb4niy/pdb
Related	4NIV 4NIW 4NIX
Descriptor	Cationic trypsin, Ecotin, CALCIUM ION, ... (5 entities in total)
Functional Keywords	trypsin inhibitor, serine proteinase, enzyme design, activation domain, peptide ligation, reverse proteolysis, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
Biological source	Bos taurus (bovine,cow,domestic cattle,domestic cow) More
Cellular location	Secreted, extracellular space: P00760 Periplasm: P23827
Total number of polymer chains	8
Total formula weight	158557.26
Authors	Schoepfel, M.,Parthier, C.,Stubbs, M.T. (deposition date: 2013-11-08, release date: 2014-02-19, Last modification date: 2014-03-19)
Primary citation	Liebscher, S.,Schopfel, M.,Aumuller, T.,Sharkhuukhen, A.,Pech, A.,Hoss, E.,Parthier, C.,Jahreis, G.,Stubbs, M.T.,Bordusa, F. N-terminal protein modification by substrate-activated reverse proteolysis. Angew.Chem.Int.Ed.Engl., 53:3024-3028, 2014 Cited by PubMed: 24520050 DOI: 10.1002/anie.201307736 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.84 Å)

Structure validation