Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4NIY

Crystal structure of trypsiligase (K60E/N143H/Y151H/D189K trypsin) complexed to YRH-ecotin (M84Y/M85R/A86H ecotin)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004175	molecular_function	endopeptidase activity
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0006508	biological_process	proteolysis
A	0007586	biological_process	digestion
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding
A	0097180	cellular_component	serine protease inhibitor complex
A	0097655	molecular_function	serpin family protein binding
B	0004175	molecular_function	endopeptidase activity
B	0004252	molecular_function	serine-type endopeptidase activity
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0006508	biological_process	proteolysis
B	0007586	biological_process	digestion
B	0008233	molecular_function	peptidase activity
B	0008236	molecular_function	serine-type peptidase activity
B	0016787	molecular_function	hydrolase activity
B	0046872	molecular_function	metal ion binding
B	0097180	cellular_component	serine protease inhibitor complex
B	0097655	molecular_function	serpin family protein binding
C	0004175	molecular_function	endopeptidase activity
C	0004252	molecular_function	serine-type endopeptidase activity
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0006508	biological_process	proteolysis
C	0007586	biological_process	digestion
C	0008233	molecular_function	peptidase activity
C	0008236	molecular_function	serine-type peptidase activity
C	0016787	molecular_function	hydrolase activity
C	0046872	molecular_function	metal ion binding
C	0097180	cellular_component	serine protease inhibitor complex
C	0097655	molecular_function	serpin family protein binding
D	0004175	molecular_function	endopeptidase activity
D	0004252	molecular_function	serine-type endopeptidase activity
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0006508	biological_process	proteolysis
D	0007586	biological_process	digestion
D	0008233	molecular_function	peptidase activity
D	0008236	molecular_function	serine-type peptidase activity
D	0016787	molecular_function	hydrolase activity
D	0046872	molecular_function	metal ion binding
D	0097180	cellular_component	serine protease inhibitor complex
D	0097655	molecular_function	serpin family protein binding
E	0004867	molecular_function	serine-type endopeptidase inhibitor activity
E	0005515	molecular_function	protein binding
E	0006952	biological_process	defense response
E	0030288	cellular_component	outer membrane-bounded periplasmic space
E	0030414	molecular_function	peptidase inhibitor activity
E	0042597	cellular_component	periplasmic space
E	0042803	molecular_function	protein homodimerization activity
F	0004867	molecular_function	serine-type endopeptidase inhibitor activity
F	0005515	molecular_function	protein binding
F	0006952	biological_process	defense response
F	0030288	cellular_component	outer membrane-bounded periplasmic space
F	0030414	molecular_function	peptidase inhibitor activity
F	0042597	cellular_component	periplasmic space
F	0042803	molecular_function	protein homodimerization activity
G	0004867	molecular_function	serine-type endopeptidase inhibitor activity
G	0005515	molecular_function	protein binding
G	0006952	biological_process	defense response
G	0030288	cellular_component	outer membrane-bounded periplasmic space
G	0030414	molecular_function	peptidase inhibitor activity
G	0042597	cellular_component	periplasmic space
G	0042803	molecular_function	protein homodimerization activity
H	0004867	molecular_function	serine-type endopeptidase inhibitor activity
H	0005515	molecular_function	protein binding
H	0006952	biological_process	defense response
H	0030288	cellular_component	outer membrane-bounded periplasmic space
H	0030414	molecular_function	peptidase inhibitor activity
H	0042597	cellular_component	periplasmic space
H	0042803	molecular_function	protein homodimerization activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 301

Chain	Residue
A	GLU70
A	ASN72
A	VAL75
A	GLU77
A	GLU80

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA B 301

Chain	Residue
B	GLU80
B	GLU70
B	ASN72
B	VAL75
B	GLU77

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ZN B 302

Chain	Residue
B	HIS143
B	HIS151
F	HIS86

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA C 301

Chain	Residue
C	GLU70
C	ASN72
C	VAL75
C	GLU77
C	GLU80

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA D 301

Chain	Residue
D	GLU70
D	ASN72
D	VAL75
D	GLU77
D	GLU80

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. VSAAHC

Chain	Residue	Details
A	VAL53-CYS58

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	Active site: {"description":"Charge relay system"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	27
Details	Binding site: {}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Site: {"description":"Reactive bond"}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)