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4NIY

Crystal structure of trypsiligase (K60E/N143H/Y151H/D189K trypsin) complexed to YRH-ecotin (M84Y/M85R/A86H ecotin)

Functional Information from GO Data
ChainGOidnamespacecontents
A0004175molecular_functionendopeptidase activity
A0004252molecular_functionserine-type endopeptidase activity
A0005515molecular_functionprotein binding
A0005576cellular_componentextracellular region
A0005615cellular_componentextracellular space
A0006508biological_processproteolysis
A0007586biological_processdigestion
A0008236molecular_functionserine-type peptidase activity
A0046872molecular_functionmetal ion binding
A0097180cellular_componentserine protease inhibitor complex
A0097655molecular_functionserpin family protein binding
B0004175molecular_functionendopeptidase activity
B0004252molecular_functionserine-type endopeptidase activity
B0005515molecular_functionprotein binding
B0005576cellular_componentextracellular region
B0005615cellular_componentextracellular space
B0006508biological_processproteolysis
B0007586biological_processdigestion
B0008236molecular_functionserine-type peptidase activity
B0046872molecular_functionmetal ion binding
B0097180cellular_componentserine protease inhibitor complex
B0097655molecular_functionserpin family protein binding
C0004175molecular_functionendopeptidase activity
C0004252molecular_functionserine-type endopeptidase activity
C0005515molecular_functionprotein binding
C0005576cellular_componentextracellular region
C0005615cellular_componentextracellular space
C0006508biological_processproteolysis
C0007586biological_processdigestion
C0008236molecular_functionserine-type peptidase activity
C0046872molecular_functionmetal ion binding
C0097180cellular_componentserine protease inhibitor complex
C0097655molecular_functionserpin family protein binding
D0004175molecular_functionendopeptidase activity
D0004252molecular_functionserine-type endopeptidase activity
D0005515molecular_functionprotein binding
D0005576cellular_componentextracellular region
D0005615cellular_componentextracellular space
D0006508biological_processproteolysis
D0007586biological_processdigestion
D0008236molecular_functionserine-type peptidase activity
D0046872molecular_functionmetal ion binding
D0097180cellular_componentserine protease inhibitor complex
D0097655molecular_functionserpin family protein binding
E0004867molecular_functionserine-type endopeptidase inhibitor activity
E0005515molecular_functionprotein binding
E0006952biological_processdefense response
E0010466biological_processnegative regulation of peptidase activity
E0030288cellular_componentouter membrane-bounded periplasmic space
E0042597cellular_componentperiplasmic space
E0042803molecular_functionprotein homodimerization activity
F0004867molecular_functionserine-type endopeptidase inhibitor activity
F0005515molecular_functionprotein binding
F0006952biological_processdefense response
F0010466biological_processnegative regulation of peptidase activity
F0030288cellular_componentouter membrane-bounded periplasmic space
F0042597cellular_componentperiplasmic space
F0042803molecular_functionprotein homodimerization activity
G0004867molecular_functionserine-type endopeptidase inhibitor activity
G0005515molecular_functionprotein binding
G0006952biological_processdefense response
G0010466biological_processnegative regulation of peptidase activity
G0030288cellular_componentouter membrane-bounded periplasmic space
G0042597cellular_componentperiplasmic space
G0042803molecular_functionprotein homodimerization activity
H0004867molecular_functionserine-type endopeptidase inhibitor activity
H0005515molecular_functionprotein binding
H0006952biological_processdefense response
H0010466biological_processnegative regulation of peptidase activity
H0030288cellular_componentouter membrane-bounded periplasmic space
H0042597cellular_componentperiplasmic space
H0042803molecular_functionprotein homodimerization activity
Functional Information from PDB Data
site_idAC1
Number of Residues5
DetailsBINDING SITE FOR RESIDUE CA A 301
ChainResidue
AGLU70
AASN72
AVAL75
AGLU77
AGLU80

site_idAC2
Number of Residues5
DetailsBINDING SITE FOR RESIDUE CA B 301
ChainResidue
BGLU80
BGLU70
BASN72
BVAL75
BGLU77

site_idAC3
Number of Residues3
DetailsBINDING SITE FOR RESIDUE ZN B 302
ChainResidue
BHIS143
BHIS151
FHIS86

site_idAC4
Number of Residues5
DetailsBINDING SITE FOR RESIDUE CA C 301
ChainResidue
CGLU70
CASN72
CVAL75
CGLU77
CGLU80

site_idAC5
Number of Residues5
DetailsBINDING SITE FOR RESIDUE CA D 301
ChainResidue
DGLU70
DASN72
DVAL75
DGLU77
DGLU80

Functional Information from PROSITE/UniProt
site_idPS00134
Number of Residues6
DetailsTRYPSIN_HIS Serine proteases, trypsin family, histidine active site. VSAAHC
ChainResidueDetails
AVAL53-CYS58

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsSITE: Reactive bond
ChainResidueDetails
ETYR84
DHIS57
DASP102
DSER195
FTYR84
GTYR84
HTYR84
BASP102
BSER195
CHIS57
CASP102
CSER195

site_idSWS_FT_FI2
Number of Residues28
DetailsBINDING:
ChainResidueDetails
AGLU70
BVAL75
BGLU80
BLYS189
BGLN192
BSER195
CGLU70
CASN72
CVAL75
CGLU80
CLYS189
AASN72
CGLN192
CSER195
DGLU70
DASN72
DVAL75
DGLU80
DLYS189
DGLN192
DSER195
AVAL75
AGLU80
ALYS189
AGLN192
ASER195
BGLU70
BASN72

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PDB entries from 2024-10-30

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