4NIY
Crystal structure of trypsiligase (K60E/N143H/Y151H/D189K trypsin) complexed to YRH-ecotin (M84Y/M85R/A86H ecotin)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Cationic trypsin | polymer | 223 | 23337.3 | 4 | UniProt (P00760) Pfam (PF00089) | Bos taurus (bovine cow domestic cattle domestic cow) | Beta-trypsin, Alpha-trypsin chain 1, Alpha-trypsin chain 2 |
| 2 | E, F, G, H (E, F, G, H) | Ecotin | polymer | 142 | 16245.6 | 4 | UniProt (P23827) Pfam (PF03974) | Escherichia coli | |
| 3 | I, J, L, M (A, B, C, D) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 4 | K (B) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 5 | N, O, P, Q, R... (A, B, C, D, E...) | water | water | 18.0 | 33 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 16 - 245 (UniProt: P00760)
E, F, G, H: 1 - 142 (UniProt: P23827)
| PDB | External Database | Details |
|---|---|---|
| Glu 60 | Lys 66 | engineered mutation |
| His 143 | Asn 146 | engineered mutation |
| His 151 | Tyr 154 | engineered mutation |
| Lys 189 | Asp 194 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Tyr 84 | Met 104 | engineered mutation |
| Arg 85 | Met 105 | engineered mutation |
| His 86 | Ala 106 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 158331.5 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 225.7 | |
| All* | Total formula weight | 158557.3 |
