Crystal structure of Schizosaccharomyces pombe AMSH-like protease sst2 catalytic domain bound to ubiquitin

Summary for 4MSQ

Related3RZU 4JXE 4MS7 4MSD 4MSJ 4MSM
DescriptorAMSH-like protease sst2, Ubiquitin, ZINC ION, ... (6 entities in total)
Functional Keywordsubiquitin, deubiquitination, helix-beta-helix sandwich, zinc metalloprotease, lysine 63-linked polyubiquitin, cytosol, endosome, hydrolase-protein binding complex, hydrolase/protein binding
Biological sourceSchizosaccharomyces pombe (Fission yeast)
Cellular locationCytoplasm Q9P371
Ubiquitin: Cytoplasm (By similarity) P0CG48
Total number of polymer chains4
Total molecular weight63103.52
Shrestha, R.K.,Ronau, J.A.,Das, C. (deposition date: 2013-09-18, release date: 2014-06-18)
Primary citation
Shrestha, R.K.,Ronau, J.A.,Davies, C.W.,Guenette, R.G.,Strieter, E.R.,Paul, L.N.,Das, C.
Insights into the Mechanism of Deubiquitination by JAMM Deubiquitinases from Cocrystal Structures of the Enzyme with the Substrate and Product.
Biochemistry, 53:3199-3217, 2014
PubMed: 24787148 (PDB entries with the same primary citation)
DOI: 10.1021/bi5003162
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.209400.9%5.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report