4MSQ

Crystal structure of Schizosaccharomyces pombe AMSH-like protease sst2 catalytic domain bound to ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	AMSH-like protease sst2	polymer	197	21981.7	2	UniProt (Q9P371) Pfam (PF01398)	Schizosaccharomyces pombe (Fission yeast)	Suppressor of ste12 deletion protein 2
2	B, D (B, D)	Ubiquitin	polymer	81	8988.3	2	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (human)
3	E, F, N, O (A, C)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
4	G, H, I, J, K... (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	13	Chemie (EDO)
5	P (C)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6	W, X, Y, Z (A, B, C, D)	water	water		18.0	305	Chemie (HOH)

Sequence modifications

A, C: 245 - 435 (UniProt: Q9P371)

PDB	External Database	Details
Gly 239	-	expression tag
Pro 240	-	expression tag
Leu 241	-	expression tag
Gly 242	-	expression tag
Ser 243	-	expression tag
Met 244	-	expression tag

B, D: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	61940.0
Non-Polymers*	Number of molecules	18
	Total formula weight	1163.5
All*	Total formula weight	63103.5

*Water molecules are not included.