4M8T

RSK2 T493M C-Terminal Kinase Domain in complex with 3-(3-(1H-pyrazol-4-yl)phenyl)-2-cyanoacrylamide

Summary for 4M8T

• Entry DOI: 10.2210/pdb4m8t/pdb
• Related: 4MAO
• Descriptor: Ribosomal protein S6 kinase alpha-3, (2E)-2-cyano-3-[3-(1H-pyrazol-4-yl)phenyl]prop-2-enamide, SODIUM ION (3 entities in total)
• Functional Keywords: protein kinase, phosphorylation, covalent inhibitor, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• Biological source: Mus musculus (mouse)
• Cellular location: Nucleus : P18654
• Total number of polymer chains: 1
• Total formula weight: 40358.88

Authors

Miller, R.M.,Taunton, J. (deposition date: 2013-08-13, release date: 2014-10-22, Last modification date: 2024-11-06)

Primary citation

London, N.,Miller, R.M.,Krishnan, S.,Uchida, K.,Irwin, J.J.,Eidam, O.,Gibold, L.,Cimermancic, P.,Bonnet, R.,Shoichet, B.K.,Taunton, J.
Covalent docking of large libraries for the discovery of chemical probes.
Nat.Chem.Biol., 10:1066-1072, 2014

PubMed Abstract: Chemical probes that form a covalent bond with a protein target often show enhanced selectivity, potency and utility for biological studies. Despite these advantages, protein-reactive compounds are usually avoided in high-throughput screening campaigns. Here we describe a general method (DOCKovalent) for screening large virtual libraries of electrophilic small molecules. We apply this method prospectively to discover reversible covalent fragments that target distinct protein nucleophiles, including the catalytic serine of AmpC β-lactamase and noncatalytic cysteines in RSK2, MSK1 and JAK3 kinases. We identify submicromolar to low-nanomolar hits with high ligand efficiency, cellular activity and selectivity, including what are to our knowledge the first reported reversible covalent inhibitors of JAK3. Crystal structures of inhibitor complexes with AmpC and RSK2 confirm the docking predictions and guide further optimization. As covalent virtual screening may have broad utility for the rapid discovery of chemical probes, we have made the method freely available through an automated web server (http://covalent.docking.org/).

PubMed: 25344815
DOI: 10.1038/nchembio.1666

Experimental method

X-RAY DIFFRACTION (3 Å)