4LXR

Structure of the Toll - Spatzle complex, a molecular hub in Drosophila development and innate immunity

4LXR の概要

• エントリーDOI: 10.2210/pdb4lxr/pdb
• 関連するPDBエントリー: 3e07 4LXS
• 分子名称: Protein toll, Protein spaetzle C-106, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: tlr, leucine-rich repeat, immune system, cytokine receptor, embryonic development, innate immunity, receptor-ligand complex', immune system-cytokine complex, immune system/cytokine
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 119557.35

構造登録者

Stelter, M.,Parthier, C.,Breithaupt, C.,Stubbs, M.T. (登録日: 2013-07-30, 公開日: 2014-04-09, 最終更新日: 2024-10-16)

主引用文献

Parthier, C.,Stelter, M.,Ursel, C.,Fandrich, U.,Lilie, H.,Breithaupt, C.,Stubbs, M.T.
Structure of the Toll-Spatzle complex, a molecular hub in Drosophila development and innate immunity.
Proc.Natl.Acad.Sci.USA, 111:6281-6286, 2014

PubMed Abstract: Drosophila Toll receptors are involved in embryonic development and the immune response of adult flies. In both processes, the only known Toll receptor ligand is the human nerve growth factor-like cystine knot protein Spätzle. Here we present the crystal structure of a 1:1 (nonsignaling) complex of the full-length Toll receptor ectodomain (ECD) with the Spätzle cystine knot domain dimer. The ECD is divided into two leucine-rich repeat (LRR) domains, each of which is capped by cysteine-rich domains. Spätzle binds to the concave surface of the membrane-distal LRR domain, in contrast to the flanking ligand interactions observed for mammalian Toll-like receptors, with asymmetric contributions from each Spätzle protomer. The structure allows rationalization of existing genetic and biochemical data and provides a framework for targeting the immune systems of insects of economic importance, as well as a variety of invertebrate disease vectors.

PubMed: 24733933
DOI: 10.1073/pnas.1320678111

実験手法

X-RAY DIFFRACTION (2.2 Å)