4LXR
Structure of the Toll - Spatzle complex, a molecular hub in Drosophila development and innate immunity
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184, 1.8448, 1.8454 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 172.025, 78.175, 124.450 |
Unit cell angles | 90.00, 126.30, 90.00 |
Refinement procedure
Resolution | 42.684 - 2.200 |
R-factor | 0.1779 |
Rwork | 0.176 |
R-free | 0.21760 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.137 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLOMON |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
High resolution limit [Å] | 2.200 | 6.530 | 2.200 |
Rmerge | 0.051 | 0.017 | 0.690 |
Number of reflections | 67514 | ||
<I/σ(I)> | 15.02 | 47.27 | 1.68 |
Completeness [%] | 98.9 | 95.8 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 10% PEG 3350, 50 mM HEPES-Na salt, pH 7.5, vapor diffusion, hanging drop, temperature 287K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 10% PEG 3350, 50 mM HEPES-Na salt, pH 7.5, vapor diffusion, hanging drop, temperature 287K |