4L55

X-ray structure of the adduct between bovine pancreatic ribonuclease and AziRu

4L55 の概要

• エントリーDOI: 10.2210/pdb4l55/pdb
• 関連するPDBエントリー: 1JVT 1JVU 1JVV 4J1A 4J1B 4LFP 4LFX 4LGK
• 分子名称: Ribonuclease pancreatic, RUTHENIUM ION (3 entities in total)
• 機能のキーワード: hydrolase, rna binding
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27618.79

構造登録者

Vergara, A.,Russo Krauss, I.,Merlino, A. (登録日: 2013-06-10, 公開日: 2014-01-29, 最終更新日: 2024-10-09)

主引用文献

Vergara, A.,Russo Krauss, I.,Montesarchio, D.,Paduano, L.,Merlino, A.
Investigating the ruthenium metalation of proteins: X-ray structure and Raman microspectroscopy of the complex between RNase A and AziRu.
Inorg.Chem., 52:10714-10716, 2013

PubMed Abstract: A Raman-assisted crystallographic study on the adduct between AziRu, a Ru(III) complex with high antiproliferative activity, and RNase A is presented. The protein structure is not perturbed significantly by the Ru label. The metal coordinates to ND atoms of His105 or of His119 imidazole rings, losing all of its original ligands but retaining octahedral, although distorted, coordination geometry. The AziRu binding inactivates the enzyme, suggesting that its antitumor action can be exerted by a mechanism of competitive inhibition.

PubMed: 24093479
DOI: 10.1021/ic401494v

実験手法

X-RAY DIFFRACTION (1.65 Å)