4J2X

CSL (RBP-Jk) with corepressor KyoT2 bound to DNA

Summary for 4J2X

• Entry DOI: 10.2210/pdb4j2x/pdb
• Related: 1TTU 2FO1 3BRD 3BRF 3BRG 3IAG
• Descriptor: Recombining binding protein suppressor of hairless, Four and a half LIM domains protein 1, 5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3', ... (6 entities in total)
• Functional Keywords: lim domain containing protein, transcription factor corepressor, dna binding, nucleus, transcription-dna binding protein-dna complex, transcription/dna binding protein/dna
• Biological source: Mus musculus (mouse); More
• Cellular location: Nucleus: P31266; Isoform 1: Cytoplasm. Isoform 2: Nucleus: P97447
• Total number of polymer chains: 8
• Total formula weight: 120724.72

Authors

Collins, K.J.,Kovall, R.A. (deposition date: 2013-02-05, release date: 2013-11-06, Last modification date: 2023-09-20)

Primary citation

Collins, K.J.,Yuan, Z.,Kovall, R.A.
Structure and Function of the CSL-KyoT2 Corepressor Complex: A Negative Regulator of Notch Signaling.
Structure, 22:70-81, 2014

PubMed Abstract: Notch refers to a highly conserved cell-to-cell signaling pathway with essential roles in embryonic development and tissue maintenance. Dysfunctional signaling causes human disease, highlighting the importance of pathway regulation. Notch signaling ultimately results in the activation of target genes, which is regulated by the nuclear effector CSL (CBF-1/RBP-J, Su(H), Lag-1). CSL dually functions as an activator and a repressor of transcription through differential interactions with coactivator or corepressor proteins, respectively. Although the structures of CSL-coactivator complexes have been determined, the structures of CSL-corepressor complexes are unknown. Here, using a combination of structural, biophysical, and cellular approaches, we characterize the structure and function of CSL in complex with the corepressor KyoT2. Collectively, our studies provide molecular insights into how KyoT2 binds CSL with high affinity and competes with coactivators, such as Notch, for binding CSL. These studies are important for understanding how CSL functions as both an activator and a repressor of transcription of Notch target genes.

PubMed: 24290140
DOI: 10.1016/j.str.2013.10.010

Experimental method

X-RAY DIFFRACTION (2.85 Å)