4J2X
CSL (RBP-Jk) with corepressor KyoT2 bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.157, 97.293, 144.102 |
| Unit cell angles | 90.00, 93.17, 90.00 |
Refinement procedure
| Resolution | 40.300 - 2.850 |
| R-factor | 0.1905 |
| Rwork | 0.188 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iag |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 2.930 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.053 | |
| Number of reflections | 37773 | |
| <I/σ(I)> | 13.9 | 1.82 |
| Completeness [%] | 95.9 | 93.96 |
| Redundancy | 5.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.1 | 277 | 21% PEG3350, 0.1 M HEPES, pH 7.1, 0.25 M ammonium acetate, MICROBATCH, temperature 277K |
