4I5Z

Insulin protein crystallization via langmuir-blodgett

Summary for 4I5Z

• Entry DOI: 10.2210/pdb4i5z/pdb
• Related: 2BN1 4I5Y
• Descriptor: Insulin (3 entities in total)
• Functional Keywords: thin films, optimal crystallization, space grown, langmuir blodgett, hormone
• Biological source: Bos taurus (bovine,cow,domestic cattle,domestic cow); More
• Cellular location: Secreted: P01317 P01317
• Total number of polymer chains: 2
• Total formula weight: 5743.57

Authors

Belmonte, L.,Pechkova, E.,Bragazzi, N.,Nicolini, C. (deposition date: 2012-11-29, release date: 2013-11-20, Last modification date: 2024-10-30)

Primary citation

Pechkova, E.,Bragazzi, N.,Bozdaganyan, M.,Belmonte, L.,Nicolini, C.
A review of the strategies for obtaining high-quality crystals utilizing nanotechnologies and microgravity
Crit Rev Eukaryot Gene Expr, 24:325-339, 2014

PubMed Abstract: Crystallization is a highly demanding and time-consuming task that causes a real bottle-neck in basic research. Great effort has been made to understand the factors and parameters that influence this process and to finely tune them to facilitate crystal growth. Different crystallization techniques have been proposed over the past decades, such as the classical vapor hanging drop method, its variant the sitting drop method, dialysis, cryo-temperature, gel, batch, and the innovative microgravity (space) techniques like free interface diffusion (FID) and counter-ion diffusion (CID). Here, we present a review of the strategies utilizing Langmuir-Blodgett (LB)-based nanotechnologies, and microgravity techniques for obtaining optimal high-quality crystals, as proven by molecular dynamics (MD) and bioinformatics approaches, namely using a clustering algorithm and protein alignment.

PubMed: 25403962
DOI: 10.1615/CritRevEukaryotGeneExpr.2014008275

Experimental method

X-RAY DIFFRACTION (1.8 Å)