4I5Z

Insulin protein crystallization via langmuir-blodgett

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Wavelength(s)	0.932
Spacegroup name	I 21 3
Unit cell lengths	78.220, 78.220, 78.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.310 - 1.800
R-factor	0.1813
Rwork	0.179
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.029
RMSD bond angle	2.207
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.310	39.110	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge		0.059	0.251
Rmeas		0.061	0.257
Rpim		0.014	0.055
Total number of observations		5303	24122
Number of reflections	7549
<I/σ(I)>	28.3	40.2	13.3
Completeness [%]	100.0	99.6	100
Redundancy	21.6	20.1	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1