Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

4HN5

GR DNA Binding Domain - TSLP nGRE Complex

Summary for 4HN5
Entry DOI10.2210/pdb4hn5/pdb
Related4HN6
DescriptorGlucocorticoid receptor, DNA (5'-D(*CP*GP*CP*CP*TP*CP*CP*GP*GP*GP*AP*GP*AP*GP*CP*T)-3'), DNA (5'-D(*AP*GP*CP*TP*CP*TP*CP*CP*CP*GP*GP*AP*GP*GP*CP*G)-3'), ... (5 entities in total)
Functional Keywordsglucocorticoid receptor, steroid receptors, dna, ngre, repression, transcription, transcription-dna complex, transcription/dna
Biological sourceHomo sapiens (human)
More
Cellular locationCytoplasm. Isoform Beta: Nucleus: P04150
Total number of polymer chains4
Total formula weight36126.09
Authors
Hudson, W.H.,Youn, C.E.,Ortlund, E.A. (deposition date: 2012-10-18, release date: 2012-12-12, Last modification date: 2024-02-28)
Primary citationHudson, W.H.,Youn, C.,Ortlund, E.A.
The structural basis of direct glucocorticoid-mediated transrepression.
Nat.Struct.Mol.Biol., 20:53-58, 2013
Cited by
PubMed Abstract: A newly discovered negative glucocorticoid response element (nGRE) mediates DNA-dependent transrepression by the glucocorticoid receptor (GR) across the genome and has a major role in immunosuppressive therapy. The nGRE differs dramatically from activating response elements, and the mechanism driving GR binding and transrepression is unknown. To unravel the mechanism of nGRE-mediated transrepression by the GR, we characterized the interaction between GR and an nGRE in the thymic stromal lymphopoietin (TSLP) promoter. We show using structural and mechanistic approaches that nGRE binding is a new mode of sequence recognition by human GR and that nGREs prevent receptor dimerization through a unique GR-binding orientation and strong negative cooperativity, ensuring the presence of monomeric GR at repressive elements.
PubMed: 23222642
DOI: 10.1038/nsmb.2456
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.902 Å)
Structure validation

229183

PDB entries from 2024-12-18

PDB statisticsPDBj update infoContact PDBjnumon