4HEL

Crystal structure analysis of apo-GroEL structure

> Summary

Summary for 4HEL

Descriptor60 kDa chaperonin 4
Functional Keywordsgroel, assist in protein folding, groes, chaperone
Biological sourceEscherichia coli
Cellular locationCytoplasm (By similarity) Q548M1
Total number of polymer chains14
Total molecular weight774897.65
Authors
Saxena, A.K.,Meena, S.R. (deposition date: 2012-10-04, release date: 2013-09-25)
Primary citation
Meena, S.R.,Saxena, A.K.
Crystal structure of apo-GroEL structure
To be Published,
Experimental method
X-RAY DIFFRACTION (3.2 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.190131.8%3.7%0.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4hel
no rotation
Molmil generated image of 4hel
rotated about x axis by 90°
Molmil generated image of 4hel
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
A, B, C, D, E...60 kDa chaperonin 4polymer52555349.814
UniProt (Q548M1)
Pfam (PF00118)
Escherichia coli@PDBjGroEL protein 4, Protein Cpn60 4
waterwater18.0901

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains14
Total molecular weight774897.6
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight774897.6
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (3.2 Å)

Cell axes136.240261.830282.280
Cell angles90.0090.0090.00
SpacegroupP 21 21 21
Resolution limits59.38 - 3.20
the highest resolution shell value3.283 - 3.200
R-factor0.19927
R-work0.19799
the highest resolution shell value0.280
R-free0.22333
the highest resolution shell value0.312
RMSD bond length0.012
RMSD bond angle1.416

Data Collection Statistics

Resolution limits59.40 - 3.20
the highest resolution shell value -
Number of reflections166588
Rmerge_l_obs0.320
the highest resolution shell value0.850
Completeness100.0
Redundancy7.5
the highest resolution shell value7.3
I/sigma(I)0

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, HANGING DROP7.5277

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
A0005737cellular_componentcytoplasm
A0005524molecular_functionATP binding
A0016491molecular_functionoxidoreductase activity
A0051082molecular_functionunfolded protein binding
A0042026biological_processprotein refolding
B0005737cellular_componentcytoplasm
B0005524molecular_functionATP binding
B0016491molecular_functionoxidoreductase activity
B0051082molecular_functionunfolded protein binding
B0042026biological_processprotein refolding
C0005737cellular_componentcytoplasm
C0005524molecular_functionATP binding
C0016491molecular_functionoxidoreductase activity
C0051082molecular_functionunfolded protein binding
C0042026biological_processprotein refolding
D0005737cellular_componentcytoplasm
D0005524molecular_functionATP binding
D0016491molecular_functionoxidoreductase activity
D0051082molecular_functionunfolded protein binding
D0042026biological_processprotein refolding
E0005737cellular_componentcytoplasm
E0005524molecular_functionATP binding
E0016491molecular_functionoxidoreductase activity
E0051082molecular_functionunfolded protein binding
E0042026biological_processprotein refolding
F0005737cellular_componentcytoplasm
F0005524molecular_functionATP binding
F0016491molecular_functionoxidoreductase activity
F0051082molecular_functionunfolded protein binding
F0042026biological_processprotein refolding
G0005737cellular_componentcytoplasm
G0005524molecular_functionATP binding
G0016491molecular_functionoxidoreductase activity
G0051082molecular_functionunfolded protein binding
G0042026biological_processprotein refolding
H0005737cellular_componentcytoplasm
H0005524molecular_functionATP binding
H0016491molecular_functionoxidoreductase activity
H0051082molecular_functionunfolded protein binding
H0042026biological_processprotein refolding
I0005737cellular_componentcytoplasm
I0005524molecular_functionATP binding
I0016491molecular_functionoxidoreductase activity
I0051082molecular_functionunfolded protein binding
I0042026biological_processprotein refolding
J0005737cellular_componentcytoplasm
J0005524molecular_functionATP binding
J0016491molecular_functionoxidoreductase activity
J0051082molecular_functionunfolded protein binding
J0042026biological_processprotein refolding
K0005737cellular_componentcytoplasm
K0005524molecular_functionATP binding
K0016491molecular_functionoxidoreductase activity
K0051082molecular_functionunfolded protein binding
K0042026biological_processprotein refolding
L0005737cellular_componentcytoplasm
L0005524molecular_functionATP binding
L0016491molecular_functionoxidoreductase activity
L0051082molecular_functionunfolded protein binding
L0042026biological_processprotein refolding
M0005737cellular_componentcytoplasm
M0005524molecular_functionATP binding
M0016491molecular_functionoxidoreductase activity
M0051082molecular_functionunfolded protein binding
M0042026biological_processprotein refolding
N0005737cellular_componentcytoplasm
N0005524molecular_functionATP binding
N0016491molecular_functionoxidoreductase activity
N0051082molecular_functionunfolded protein binding
N0042026biological_processprotein refolding
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
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Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails
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Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails
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Catalytic Information from CSA

site_idNumber of ResiduesDetails

> Sequence Neighbor

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