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4HEL

Crystal structure analysis of apo-GroEL structure

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE BM14
Synchrotron siteESRF
BeamlineBM14
Temperature [K]100
Detector technologyCCD
Collection date2011-11-09
DetectorMARCCD225
Wavelength(s)0.97327
Spacegroup nameP 21 21 21
Unit cell lengths136.240, 261.830, 282.280
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution59.380 - 3.200
R-factor0.19927
Rwork0.198
R-free0.22333
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1kp8
RMSD bond length0.012
RMSD bond angle1.416
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwarePHASER
Refinement softwareREFMAC (5.5.0072)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]59.4003.370
High resolution limit [Å]3.2003.200
Rmerge0.3200.850
Number of reflections166588
<I/σ(I)>6.12.4
Completeness [%]100.0100
Redundancy7.57.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.527732% MPD, 100mM Tris-HCl, 160mM MgCl2, 10% Glycerol, 2%(w/v) PEG6000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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