4H54

Crystal structure of the diguanylate cyclase DgcZ

Summary for 4H54

• Entry DOI: 10.2210/pdb4h54/pdb
• Related: 3T9O 3TVK
• Descriptor: Diguanylate cyclase YdeH, ZINC ION, GUANOSINE-5'-RP-ALPHA-THIO-TRIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: zinc sensor, c-di-gmp, czb domain, transferase
• Biological source: Escherichia coli
• Total number of polymer chains: 2
• Total formula weight: 72795.67

Authors

Zaehringer, F.,Schirmer, T. (deposition date: 2012-09-18, release date: 2013-07-03, Last modification date: 2023-09-20)

Primary citation

Zahringer, F.,Lacanna, E.,Jenal, U.,Schirmer, T.,Boehm, A.
Structure and signaling mechanism of a zinc-sensory diguanylate cyclase.
Structure, 21:1149-1157, 2013

PubMed Abstract: Diguanylate cyclases synthesize the second messenger c-di-GMP, which in turn governs a plethora of physiological processes in bacteria. Although most diguanylate cyclases harbor sensory domains, their input signals are largely unknown. Here, we demonstrate that diguanylate cyclase DgcZ (YdeH) from Escherichia coli is regulated allosterically by zinc. Crystal structures show that the zinc ion is bound to the 3His/1Cys motif of the regulatory chemoreceptor zinc-binding domain, which mediates subunit contact within the dimeric enzyme. In vitro, zinc reversibly inhibits DgcZ with a subfemtomolar Ki constant. In vivo, bacterial biofilm formation is modulated by externally applied zinc in a DgcZ- and c-di-GMP-dependent fashion. The study outlines the structural principles of this zinc sensor. Zinc binding seems to regulate the activity of the catalytic GGDEF domains by impeding their mobility and thus preventing productive encounter of the two GTP substrates.

PubMed: 23769666
DOI: 10.1016/j.str.2013.04.026

Experimental method

X-RAY DIFFRACTION (3.9 Å)