4H54

Crystal structure of the diguanylate cyclase DgcZ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Diguanylate cyclase YdeH	polymer	304	34884.4	2	UniProt (P31129) Pfam (PF13682) Pfam (PF00990) In PDB	Escherichia coli	DgcZ, DGC
2	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	A, B	GUANOSINE-5'-RP-ALPHA-THIO-TRIPHOSPHATE	non-polymer		539.2	4	Chemie (GAV)
4	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	A	9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)	non-polymer		690.4	1	Chemie (C2E)

Sequence modifications

A, B: 2 - 296 (UniProt: P31129)

PDB	External Database	Details
Ala 1	-	expression tag
Ala 52	Cys 52	engineered mutation
Leu 297	-	expression tag
Glu 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69768.9
Non-Polymers*	Number of molecules	9
	Total formula weight	3026.8
All*	Total formula weight	72795.7

*Water molecules are not included.