4H54
Crystal structure of the diguanylate cyclase DgcZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 |
Unit cell lengths | 102.593, 102.593, 129.156 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 73.200 - 3.900 |
R-factor | 0.2421 |
Rwork | 0.242 |
R-free | 0.24110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3T90 AND 3TVK |
RMSD bond length | 0.016 |
RMSD bond angle | 1.040 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 89.000 | 89.050 | 4.110 |
High resolution limit [Å] | 3.900 | 12.330 | 3.900 |
Rmerge | 0.030 | 0.770 | |
Number of reflections | 7076 | ||
<I/σ(I)> | 15.45 | 1 | |
Completeness [%] | 99.9 | 99.06 | 99.87 |
Redundancy | 13.38 | 7.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 200 mM calcium acetate, 20.0% PEG4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |