4GSP

RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS

Summary for 4GSP

• Entry DOI: 10.2210/pdb4gsp/pdb
• Descriptor: RIBONUCLEASE T1, CALCIUM ION, GUANOSINE-3'-MONOPHOSPHATE, ... (5 entities in total)
• Functional Keywords: hydrolase, endoribonuclease
• Biological source: Aspergillus oryzae
• Total number of polymer chains: 1
• Total formula weight: 11859.26

Authors

Zegers, I.,Wyns, L. (deposition date: 1997-12-02, release date: 1998-08-12, Last modification date: 2024-10-30)

Primary citation

Zegers, I.,Loris, R.,Dehollander, G.,Fattah Haikal, A.,Poortmans, F.,Steyaert, J.,Wyns, L.
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography.
Nat.Struct.Biol., 5:280-283, 1998

PubMed Abstract: Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.

PubMed: 9546218
DOI: 10.1038/nsb0498-280

Experimental method

X-RAY DIFFRACTION (1.65 Å)