4GSP

RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	48.240, 51.100, 40.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.650
R-factor	0.159
Rwork	0.159
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rga
RMSD bond length	0.080
RMSD bond angle	24.881 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.083 *	0.518 *
Total number of observations	245273 *
Number of reflections	11810
<I/σ(I)>	33.7	9
Completeness [%]	97.2	88.4 *
Redundancy	93.1	16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.2		20 MM NAAC PH 4.2 2 MM CACL2 55 % MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNase T1	15 (mg/ml)
2	1	drop	exo-cGPS	15 (mM)
3	1	drop		20 (mM)
4	1	drop		2 (mM)
5	1	reservoir	MPD	55 (%(v/v))