4GIW

Crystal structure of the RUN domain of human NESCA

Summary for 4GIW

• Entry DOI: 10.2210/pdb4giw/pdb
• Descriptor: RUN and SH3 domain-containing protein 1 (2 entities in total)
• Functional Keywords: ngf-trka signaling pathway, nf-kb pathway, signaling protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: Q9BVN2
• Total number of polymer chains: 2
• Total formula weight: 43659.85

Authors

Bai, L.,Sun, Q.,Jiang, T. (deposition date: 2012-08-09, release date: 2012-08-29, Last modification date: 2024-11-06)

Primary citation

Sun, Q.,Han, C.,Liu, L.,Wang, Y.,Deng, H.,Bai, L.,Jiang, T.
Crystal structure and functional implication of the RUN domain of human NESCA
Protein Cell, 3:609-617, 2012

PubMed Abstract: NESCA, a newly discovered signaling adapter protein in the NGF-pathway, contains a RUN domain at its N-terminus. Here we report the crystal structure of the NESCA RUN domain determined at 2.0-Å resolution. The overall fold of the NESCA RUN domain comprises nine helices, resembling the RUN domain of RPIPx and the RUN1 domain of Rab6IP1. However, compared to the other RUN domains, the RUN domain of NESCA has significantly different surface electrostatic distributions at the putative GTPase-interacting interface. We demonstrate that the RUN domain of NESCA can bind H-Ras, a downstream signaling molecule of TrkA, with high affinity. Moreover, NESCA RUN can directly interact with TrkA. These results provide new insights into how NESCA participates in the NGF-TrkA signaling pathway.

PubMed: 22821014
DOI: 10.1007/s13238-012-2052-3

Experimental method

X-RAY DIFFRACTION (2 Å)