4GIW
Crystal structure of the RUN domain of human NESCA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.116, 94.201, 44.602 |
| Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
| Resolution | 35.700 - 2.000 |
| R-factor | 0.20488 |
| Rwork | 0.204 |
| R-free | 0.21233 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.103 |
| Number of reflections | 20352 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 99.6 | 99.42 |
| Redundancy | 7.247 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20%(w/v) PEG3350, 0.2M K2HPO4, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
