4GIE

Crystal structure of prostaglandin F synthase from Trypanosoma cruzi bound to NADP

Summary for 4GIE

• Entry DOI: 10.2210/pdb4gie/pdb
• Related: 4F40 4FZI 4G5D
• Descriptor: Prostaglandin F synthase, ACETATE ION, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total)
• Functional Keywords: structural genomics, niaid, national institute of allergy and infectious diseases, seattle structural genomics center for infectious disease, ssgcid, chagas disease, prostaglandin d2, trypanosomiasis, eukaryotic pathogen, parasite, nadp+, nadph, rutin, bimatoprost, oxidoreductase
• Biological source: Trypanosoma cruzi
• Cellular location: Cytoplasm : Q4DJ07
• Total number of polymer chains: 1
• Total formula weight: 34394.48

Authors

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2012-08-08, release date: 2012-08-15, Last modification date: 2023-09-13)

Primary citation

Moen, S.O.,Fairman, J.W.,Barnes, S.R.,Sullivan, A.,Nakazawa-Hewitt, S.,Van Voorhis, W.C.,Staker, B.L.,Lorimer, D.D.,Myler, P.J.,Edwards, T.E.
Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.
Acta Crystallogr F Struct Biol Commun, 71:609-614, 2015

PubMed Abstract: The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.

PubMed: 25945716
DOI: 10.1107/S2053230X15006883

Experimental method

X-RAY DIFFRACTION (1.25 Å)