4GCW

Crystal structure of RNase Z in complex with precursor tRNA(Thr)

4GCW の概要

• エントリーDOI: 10.2210/pdb4gcw/pdb
• 関連するPDBエントリー: 1Y44 2FK6
• 分子名称: Ribonuclease Z, TRNA(THR), ZINC ION, ... (5 entities in total)
• 機能のキーワード: zinc-dependent metalo betalactamase - rna complex, trna maturase, protein-rna complex, trna binding, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Bacillus subtilis subsp. subtilis; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60556.36

構造登録者

Pellegrini, O.,Li de la Sierra-Gallay, I.,Piton, J.,Gilet, L.,Condon, C. (登録日: 2012-07-31, 公開日: 2012-10-31, 最終更新日: 2023-09-13)

主引用文献

Pellegrini, O.,Li de la Sierra-Gallay, I.,Piton, J.,Gilet, L.,Condon, C.
Activation of tRNA Maturation by Downstream Uracil Residues in B. subtilis
Structure, 20:1769-1777, 2012

PubMed Abstract: Ribonuclease (RNase) Z is involved in the maturation of the 3' ends of transfer RNAs (tRNAs) in all three kingdoms of life. To prevent futile cycles of CCA addition and removal, eukaryotic RNase Z discriminates against mature tRNAs bearing a CCA motif, with the first cytosine residue (C74) being the key antideterminant. Here, we show that, remarkably, the B. subtilis enzyme does not discriminate against cytosine in position 74, but rather is highly stimulated by uracil in this location. Consistent with this observation, the vast majority of B. subtilis tRNA precursor substrates of RNase Z naturally contain U74. Those tRNA precursors with a uracil further downstream are also substrates for RNase Z, but are matured in a two-step endo/exonuclease reaction. We solved the first crystal structure of B. subtilis RNase Z bound to a tRNA(Thr) precursor with U74 and show that the enzyme has a specific binding pocket for this nucleotide.

PubMed: 22940585
DOI: 10.1016/j.str.2012.08.002

実験手法

X-RAY DIFFRACTION (3 Å)