4EWI

Crystal structure of the NLRP4 Pyrin domain

Summary for 4EWI

• Entry DOI: 10.2210/pdb4ewi/pdb
• Descriptor: NACHT, LRR and PYD domains-containing protein 4, CHLORIDE ION, SULFATE ION, ... (4 entities in total)
• Functional Keywords: nlr proteins, death domain, pyrin domain, nlrp4, asc, innate immune system, inflammasome, apoptosis, protein-protein interaction, signaling protein, protein binding
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 27785.20

Authors

Eibl, C.,Hessenberger, M.,Puehringer, S.,Page, R.,Diederichs, K.,Peti, W. (deposition date: 2012-04-27, release date: 2012-09-05, Last modification date: 2024-02-28)

Primary citation

Eibl, C.,Grigoriu, S.,Hessenberger, M.,Wenger, J.,Puehringer, S.,Pinheiro, A.S.,Wagner, R.N.,Proell, M.,Reed, J.C.,Page, R.,Diederichs, K.,Peti, W.
Structural and Functional Analysis of the NLRP4 Pyrin Domain.
Biochemistry, 51:7330-7341, 2012

PubMed Abstract: NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily and adopts a DD fold consisting of six α-helices tightly packed around a hydrophobic core, with a highly charged surface that is typical of PYDs. Importantly, however, we identified several differences between the NLRP4 PYD crystal structure and other PYD structures that are significant enough to affect NLRP4 function and its interactions with binding partners. Notably, the length of helix α3 and the α2-α3 connecting loop in the NLRP4 PYD are unique among PYDs. The apoptosis-associated speck-like protein containing a CARD (ASC) is an adaptor protein whose interactions with a number of distinct PYDs are believed to be critical for activation of the inflammatory response. Here, we use co-immunoprecipitation, yeast two-hybrid, and nuclear magnetic resonance chemical shift perturbation analysis to demonstrate that, despite being important for activation of the inflammatory response and sharing several similarities with other known ASC-interacting PYDs (i.e., ASC2), NLRP4 does not interact with the adaptor protein ASC. Thus, we propose that the factors governing homotypic PYD interactions are more complex than the currently accepted model, which states that complementary charged surfaces are the main determinants of PYD-PYD interaction specificity.

PubMed: 22928810
DOI: 10.1021/bi3007059

Experimental method

X-RAY DIFFRACTION (2.28 Å)