4EWI

Crystal structure of the NLRP4 Pyrin domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-05
Detector	RAYONIX MX-225
Wavelength(s)	0.91841, 0.97958, 0.97973, 0.9719
Spacegroup name	P 32 2 1
Unit cell lengths	62.107, 62.107, 124.932
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.759 - 2.280
R-factor	0.209
Rwork	0.207
R-free	0.24550
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.626
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine: dev_1009))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.760	2.420
High resolution limit [Å]	2.280	2.280
Rmerge	0.059	0.822
Number of reflections	13300
<I/σ(I)>	20.82	2.95
Completeness [%]	99.9	99.9
Redundancy	7.7	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	0.2 M Disodium tartrate, 2.2 M Ammonium sulfate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K