4ELM

Crystal structure of the mouse CD1d-lysosulfatide-Hy19.3 TCR complex

Summary for 4ELM

• Entry DOI: 10.2210/pdb4elm/pdb
• Related: 4ELK
• Descriptor: Antigen-presenting glycoprotein CD1d1, Beta-2 microglobulin, Hy19.3 TCR alpha chain (mouse variable domain, human constant domain), ... (9 entities in total)
• Functional Keywords: antigen presentation, glycolipid, nkt cells, immune system
• Biological source: Mus musculus (mouse); More
• Total number of polymer chains: 8
• Total formula weight: 194630.49

Authors

Girardi, E.,Maricic, I.,Wang, J.,Mac, T.T.,Iyer, P.,Kumar, V.,Zajonc, D.M. (deposition date: 2012-04-11, release date: 2012-07-25, Last modification date: 2024-11-06)

Primary citation

Girardi, E.,Maricic, I.,Wang, J.,Mac, T.T.,Iyer, P.,Kumar, V.,Zajonc, D.M.
Type II natural killer T cells use features of both innate-like and conventional T cells to recognize sulfatide self antigens.
Nat.Immunol., 13:851-856, 2012

PubMed Abstract: Glycolipids presented by the major histocompatibility complex (MHC) class I homolog CD1d are recognized by natural killer T cells (NKT cells) characterized by either a semi-invariant T cell antigen receptor (TCR) repertoire (type I NKT cells or iNKT cells) or a relatively variable TCR repertoire (type II NKT cells). Here we describe the structure of a type II NKT cell TCR in complex with CD1d-lysosulfatide. Both TCR α-chains and TCR β-chains made contact with the CD1d molecule with a diagonal footprint, typical of MHC-TCR interactions, whereas the antigen was recognized exclusively with a single TCR chain, similar to the iNKT cell TCR. Type II NKT cell TCRs, therefore, recognize CD1d-sulfatide complexes by a distinct recognition mechanism characterized by the TCR-binding features of both iNKT cells and conventional peptide-reactive T cells.

PubMed: 22820602
DOI: 10.1038/ni.2371

Experimental method

X-RAY DIFFRACTION (3.48 Å)