4ELM
Crystal structure of the mouse CD1d-lysosulfatide-Hy19.3 TCR complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9765 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.514, 126.996, 104.349 |
Unit cell angles | 90.00, 110.53, 90.00 |
Refinement procedure
Resolution | 92.260 - 3.480 |
R-factor | 0.21163 |
Rwork | 0.209 |
R-free | 0.26494 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.105 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0104) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.260 | 3.690 |
High resolution limit [Å] | 3.480 | 3.500 |
Rmerge | 0.240 | 0.814 |
Number of reflections | 30877 | |
<I/σ(I)> | 8.3 | 2.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.3 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 11% PEG4000, 4% tacsimate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |