4EE3

Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide

Summary for 4EE3

Related4EE4 4EE5 4EEA 4EEG 4EEM 4EEO 2AEC
DescriptorBeta-1,4-galactosyltransferase 1, BETA-D-GLUCOSE, BETA-D-GALACTOSE, ... (9 entities in total)
Functional Keywordsprotein-carbohydrate complex, gt-a fold, glycosyltransferase, transferase
Biological sourceHomo sapiens (human)
Cellular locationIsoform Long: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein. Isoform Short: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein. Processed beta-1,4-galactosyltransferase 1: Secreted  P15291
Total number of polymer chains3
Total molecular weight104948.58
Authors
Ramakrishnan, B.,Qasba, P.K. (deposition date: 2012-03-28, release date: 2012-07-04, Last modification date: 2017-11-15)
Primary citation
Ramakrishnan, B.,Boeggeman, E.,Qasba, P.K.
Binding of N-acetylglucosamine (GlcNAc) beta 1-6-branched oligosaccharide acceptors to beta 4-galactosyltransferase I reveals a new ligand binding mode.
J.Biol.Chem., 287:28666-28674, 2012
PubMed: 22740701 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M112.373514
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.3 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.235402.0%1.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4ee3
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Molmil generated image of 4ee3
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More Biological unit images

Molmil generated image of 4ee3
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Molmil generated image of 4ee3
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Molmil generated image of 4ee3
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(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (4ee3.pdb1.gz [55.02 KB])
Coordinate files for Biological unit (4ee3.pdb4.gz [305.23 KB])
Coordinate files for Biological unit (4ee3.pdb5.gz [154.95 KB])
Coordinate files for Biological unit (4ee3.pdb3.gz [54.38 KB])
Coordinate files for Biological unit (4ee3.pdb2.gz [54.86 KB])