4EE3
Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C | Beta-1,4-galactosyltransferase 1 | polymer | 287 | 32773.2 | 3 | UniProt (P15291) Pfam (PF13733) Pfam (PF02709) In PDB | Homo sapiens (human) | Beta-1,4-GalTase 1, Beta4Gal-T1, b4Gal-T1, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1, Lactose synthase A protein, N-acetyllactosamine synthase, Nal synthase, Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase, Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase, Processed beta-1,4-galactosyltransferase 1 |
2 | D, E, F | beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)]beta-D-galactopyranose-(1-4)-beta-D-glucopyranose | branched | 910.8 | 3 | In PDB GlyTouCan (G33889GK) | |||
3 | C, A, B | 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE | non-polymer | 503.3 | 3 | Chemie (UDH) | |||
4 | C, A, B | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
5 | C, A, B | SULFATE ION | non-polymer | 96.1 | 18 | Chemie (SO4) | |||
6 | C, A, B | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
7 | water | water | 18.0 | 527 | Chemie (HOH) |
Sequence modifications
A, B, C: 126 - 398 (UniProt: P15291)
PDB | External Database | Details |
---|---|---|
Ala 112 | - | expression tag |
Ser 113 | - | expression tag |
Met 114 | - | expression tag |
Thr 115 | - | expression tag |
Gly 116 | - | expression tag |
Gly 117 | - | expression tag |
Gln 118 | - | expression tag |
Gln 119 | - | expression tag |
Met 120 | - | expression tag |
Gly 121 | - | expression tag |
Arg 122 | - | expression tag |
Gly 123 | - | expression tag |
Ser 124 | - | expression tag |
Ala 125 | - | expression tag |
Thr 337 | Arg 337 | engineered mutation |
Thr 338 | Cys 338 | engineered mutation |
His 340 | Met 340 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 98319.7 | |
Branched | Number of molecules | 3 |
Total formula weight | 2732.5 | |
Non-Polymers* | Number of molecules | 27 |
Total formula weight | 3680.2 | |
All* | Total formula weight | 104732.4 |