4EE3
Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-09-09 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.612, 195.158, 143.695 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.310 - 2.300 |
R-factor | 0.18922 |
Rwork | 0.187 |
R-free | 0.24014 |
Structure solution method | Molecular placement |
Starting model (for MR) | 2aec |
RMSD bond length | 0.019 |
RMSD bond angle | 1.948 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 57756 | |
<I/σ(I)> | 17.9 | 3.3 |
Completeness [%] | 91.0 | 66.7 |
Redundancy | 6.4 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |