4EB8

Crystal structure of purine nucleoside phosphorylase (W16Y, W94Y, W178Y, H257W) mutant from human complexed with DADMe-ImmG and phosphate

Summary for 4EB8

• Entry DOI: 10.2210/pdb4eb8/pdb
• Related: 4EAR
• Descriptor: Purine nucleoside phosphorylase, PHOSPHATE ION, 2-amino-7-{[(3R,4R)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one, ... (5 entities in total)
• Functional Keywords: pnp, immucillin, purine nucleoside phosphorylase, nucleoside binding, purine base binding, purine-nucleoside phosphorylase activity, drug binding, transferase activity, transferring glycosyl groups, phosphate ion binding, intracellular, cytosol, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm, cytoskeleton (By similarity): P00491
• Total number of polymer chains: 3
• Total formula weight: 109628.32

Authors

Ho, M.C.,Haapalainen, A.M.,Suarez, J.J.,Almo, S.C.,Schramm, V.L. (deposition date: 2012-03-23, release date: 2013-02-06, Last modification date: 2024-02-28)

• Primary citation: Suarez, J.,Haapalainen, A.M.,Cahill, S.M.,Ho, M.C.,Yan, F.,Almo, S.C.,Schramm, V.L.; Catalytic Site Conformations in Human PNP by (19)F-NMR and Crystallography.; Chem.Biol., 20:212-222, 2013; PubMed: 23438750; DOI: 10.1016/j.chembiol.2013.01.009

Experimental method

X-RAY DIFFRACTION (2.3 Å)